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Addgene

pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor
(Plasmid #221832)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221832 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTwist Amp High Copy
  • Backbone manufacturer
    TWIST Bioscience
  • Vector type
    Mammalian Expression, Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    7xGFP11-HA donor with 5-HT1AR homology arms
  • Species
    D. melanogaster (fly)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13 fwd
  • 3′ sequencing primer M13 rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.14.589413 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor was a gift from Quentin Gaudry (Addgene plasmid # 221832)

  • For your References section:

    Serotonin acts through multiple cellular targets during an olfactory critical period. Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM. bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 10.1101/2024.04.14.589413. 10.1101/2024.04.14.589413 PubMed 38645269
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