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Addgene

pEBG-hBEN L38&45P
(Plasmid #22182)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22182 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBG
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hBEN
  • Alt name
    MusTRD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2900
  • Mutation
    Changed Leucine 38 to Proline and changed Leucine 45 to Proline
  • Entrez Gene
    GTF2IRD1 (a.k.a. BEN, CREAM1, GTF3, MUSTRD1, RBAP2, WBS, WBSCR11, WBSCR12, hMusTRD1alpha1)
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer EF-1a Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Restriction sites may be incorrect.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBG-hBEN L38&45P was a gift from Ananda Roy (Addgene plasmid # 22182 ; http://n2t.net/addgene:22182 ; RRID:Addgene_22182)

  • For your References section:

    Regulation of immunoglobulin promoter activity by TFII-I class transcription factors. Tantin D, Tussie-Luna MI, Roy AL, Sharp PA. J Biol Chem. 2004 Feb 13. 279(7):5460-9. 10.1074/jbc.M311177200 PubMed 14645227
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