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Addgene

Gluc-LHCGR
(Plasmid #221781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-LHCGR
  • Total vector size (bp) 6658
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LHCGR
  • Alt name
    Luteinizing Hormone/Choriogonadotropin Receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2022
  • Entrez Gene
    LHCGR (a.k.a. HHG, LCGR, LGR2, LH/CG-R, LH/CGR, LHR, LHRHR, LSH-R, ULG5)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Gaussia Luciferase (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATC
  • 3′ sequencing primer CACACCACAGAAGTAAGGTTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gluc-LHCGR was a gift from Adolfo Rivero-Muller (Addgene plasmid # 221781 ; http://n2t.net/addgene:221781 ; RRID:Addgene_221781)

  • For your References section:

    Glycoprotein-glycoprotein Receptor Binding Detection Using Bioluminescence Resonance Energy Transfer. Adamczuk K, Ngo TH, Czapinski J, Rivero-Muller A. Endocrinology. 2024 Apr 29;165(6):bqae052. doi: 10.1210/endocr/bqae052. 10.1210/endocr/bqae052 PubMed 38679471
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