MZB222
(Plasmid #221772)

• Purpose: Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases
• Depositing Lab: Richard Baker
• Publication: A structure-based mechanism for initiation of AP-3 coated vesicle formation (unpublished)

Backbone

• Vector backbone: pET29b
• Backbone size w/o insert (bp): 5612
• Total vector size (bp): 6209
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ArfGEF1
• Alt name: BIG1
• Species: H. sapiens (human)
• Insert Size (bp): 597
• Entrez Gene: ARFGEF1 (a.k.a. ARFGEP1, BIG1, DEDISB, P200)
• Promoter: T7 Promoter
• Tag / Fusion Protein:
  • 10xHis-SUMO (N terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • MZB222.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry