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Addgene

MZB218
(Plasmid #221769)

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This material is available to academics and nonprofits only. Availability may be limited outside the U.S. Please log in for more information.
Item Catalog # Description Quantity Price (USD)
Plasmid 221769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone size w/o insert (bp) 5364
  • Total vector size (bp) 6556
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Arf4
  • Alt name
    ADP-ribosylation factor 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    540
  • Mutation
    Q71L
  • Entrez Gene
    ARF4 (a.k.a. ARF2)
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • CPD-10xHis (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector encodes for a portion of the Vibrio cholerae MARTX (rtxA) toxin. A majority of the pathogenic portions of the toxin are not present, therefore regular caution should be exercised when expressing the fusion protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MZB218 was a gift from Richard Baker (Addgene plasmid # 221769 ; http://n2t.net/addgene:221769 ; RRID:Addgene_221769)
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