MZB216
(Plasmid #221766)

• Purpose: "Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
• Depositing Lab: Richard Baker
• Publication: A structure-based mechanism for initiation of AP-3 coated vesicle formation (unpublished)

Backbone

• Vector backbone: pET21b
• Backbone size w/o insert (bp): 5364
• Total vector size (bp): 6027

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: rtxA
• Alt name: Multifunctional-autoprocessing repeats-in-toxin
• Alt name: CPD
• Alt name: Cysteine protease domain
• Species: Vibrio cholerae
• Insert Size (bp): 663
• Promoter: T7 Promoter

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • MZB216.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector encodes for a portion of the Vibrio cholerae MARTX (rtxA) toxin. A majority of the pathogenic portions of the toxin are not present, therefore regular caution should be exercised when expressing the fusion protein.

"Empty" vector for rapid cloning via DNA Assembly (i.e. Gibson, HiFi, etc.) via SwaI linearization