pcDNA3-FLAG3-drAgo2D-E
(Plasmid
#221731)
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PurposeExpression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedrAgo2D-E
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SpeciesD. rerio (zebrafish)
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MutationD651E in active site
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Entrez Geneago2
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FLAG3-drAgo2D-E was a gift from David Bartel (Addgene plasmid # 221731 ; http://n2t.net/addgene:221731 ; RRID:Addgene_221731) -
For your References section:
A Seed Mismatch Enhances Argonaute2-Catalyzed Cleavage and Partially Rescues Severely Impaired Cleavage Found in Fish. Chen GR, Sive H, Bartel DP. Mol Cell. 2017 Dec 21;68(6):1095-1107.e5. doi: 10.1016/j.molcel.2017.11.032. 10.1016/j.molcel.2017.11.032 PubMed 29272705
