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Addgene

pCS2+-FLAG3-hsN-drAgo2
(Plasmid #221717)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221717 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Vector type
    Fish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsN-drAgo2
  • Species
    D. rerio (zebrafish)
  • Mutation
    Swap of N-domain for human Ago2 N-domain
  • Entrez Gene
    ago2
  • Promoter simian CMV IE94
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+-FLAG3-hsN-drAgo2 was a gift from David Bartel (Addgene plasmid # 221717 ; http://n2t.net/addgene:221717 ; RRID:Addgene_221717)

  • For your References section:

    A Seed Mismatch Enhances Argonaute2-Catalyzed Cleavage and Partially Rescues Severely Impaired Cleavage Found in Fish. Chen GR, Sive H, Bartel DP. Mol Cell. 2017 Dec 21;68(6):1095-1107.e5. doi: 10.1016/j.molcel.2017.11.032. 10.1016/j.molcel.2017.11.032 PubMed 29272705
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