pGY18
(Plasmid
#221708)
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Purpose(Empty Backbone) Empty Cas9 vector for multiplex gene editing in fungi
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFC332
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Vector typeBacterial Expression, CRISPR ; Aspergillus
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGY18 was a gift from Kyle Pomraning (Addgene plasmid # 221708 ; http://n2t.net/addgene:221708 ; RRID:Addgene_221708) -
For your References section:
CRISPR-Cas9/Cas12a systems for efficient genome editing and large genomic fragment deletions in Aspergillus niger. Yuan G, Deng S, Czajka JJ, Dai Z, Hofstad BA, Kim J, Pomraning KR. Front Bioeng Biotechnol. 2024 Oct 16;12:1452496. doi: 10.3389/fbioe.2024.1452496. eCollection 2024. 10.3389/fbioe.2024.1452496 PubMed 39479294