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Addgene

plenti-FLAG-CAMK2D-ED
(Plasmid #221700)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221700 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-C-myc-DDK-P2A-Puro
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CAMK2D
  • Species
    H. sapiens (human)
  • Mutation
    K43R, D136N
  • Entrez Gene
    CAMK2D (a.k.a. CAMKD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AsiSI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a mutated version of plasmid #221699.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plenti-FLAG-CAMK2D-ED was a gift from Derrick Sek Tong Ong (Addgene plasmid # 221700 ; http://n2t.net/addgene:221700 ; RRID:Addgene_221700)

  • For your References section:

    CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma. Chuah YH, Tay EXY, Grinchuk OV, Yoon J, Feng J, Kannan S, Robert M, Jakhar R, Liang Y, Lee BWL, Wang LC, Lim YT, Zhao T, Sobota RM, Lu G, Low BC, Crasta KC, Verma CS, Lin Z, Ong DST. Cell Death Differ. 2023 Aug;30(8):1973-1987. doi: 10.1038/s41418-023-01192-3. Epub 2023 Jul 19. 10.1038/s41418-023-01192-3 PubMed 37468549
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