plenti-FLAG-CAMK2D
(Plasmid
#221699)
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PurposeLentiviral expression of FLAG-tagged human CAMK2D in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221699 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti-C-myc-DDK-P2A-Puro
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCAMK2D
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SpeciesH. sapiens (human)
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Entrez GeneCAMK2D (a.k.a. CAMKD)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AsiSI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plenti-FLAG-CAMK2D was a gift from Derrick Sek Tong Ong (Addgene plasmid # 221699 ; http://n2t.net/addgene:221699 ; RRID:Addgene_221699) -
For your References section:
CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma. Chuah YH, Tay EXY, Grinchuk OV, Yoon J, Feng J, Kannan S, Robert M, Jakhar R, Liang Y, Lee BWL, Wang LC, Lim YT, Zhao T, Sobota RM, Lu G, Low BC, Crasta KC, Verma CS, Lin Z, Ong DST. Cell Death Differ. 2023 Aug;30(8):1973-1987. doi: 10.1038/s41418-023-01192-3. Epub 2023 Jul 19. 10.1038/s41418-023-01192-3 PubMed 37468549