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Addgene

pCMV-FLAG-CDC20
(Plasmid #221694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221694 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag2B
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDC20
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDC20 (a.k.a. CDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-FLAG-CDC20 was a gift from Derrick Sek Tong Ong (Addgene plasmid # 221694 ; http://n2t.net/addgene:221694 ; RRID:Addgene_221694)

  • For your References section:

    CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma. Chuah YH, Tay EXY, Grinchuk OV, Yoon J, Feng J, Kannan S, Robert M, Jakhar R, Liang Y, Lee BWL, Wang LC, Lim YT, Zhao T, Sobota RM, Lu G, Low BC, Crasta KC, Verma CS, Lin Z, Ong DST. Cell Death Differ. 2023 Aug;30(8):1973-1987. doi: 10.1038/s41418-023-01192-3. Epub 2023 Jul 19. 10.1038/s41418-023-01192-3 PubMed 37468549
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