Skip to main content
Addgene

pEBG-TFII-I L30&37P
(Plasmid #22166)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22166 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEBG
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TFII-I
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3300
  • Mutation
    Changed Leucine 30 to Proline and changed leucine 37 to Proline
  • Entrez Gene
    GTF2I (a.k.a. BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6)
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer EF-1a Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBG-TFII-I L30&37P was a gift from Ananda Roy (Addgene plasmid # 22166 ; http://n2t.net/addgene:22166 ; RRID:Addgene_22166)

  • For your References section:

    Mechanism of Bruton's tyrosine kinase-mediated recruitment and regulation of TFII-I. Sacristan C, Tussie-Luna MI, Logan SM, Roy AL. J Biol Chem. 2004 Feb 20. 279(8):7147-58. 10.1074/jbc.M303724200 PubMed 14623887
Related items: