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p8215 pHAGE N-V5 PTPN14 ΔPPXY1/2
(Plasmid #221646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-N-V5
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PTPN14 ΔPPXY1/2
  • Species
    H. sapiens (human)
  • Mutation
    deletion of the first and second (tandem) PPXY motifs as described in PMID: 31486770
  • Entrez Gene
    PTPN14 (a.k.a. CATLPH, PEZ, PTP36, PTPD2)
  • Tag / Fusion Protein
    • V5 (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.07.583953 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p8215 pHAGE N-V5 PTPN14 ΔPPXY1/2 was a gift from Elizabeth White (Addgene plasmid # 221646 ; http://n2t.net/addgene:221646 ; RRID:Addgene_221646)

  • For your References section:

    HPV18 E7 inhibits LATS1 kinase and activates YAP1 by degrading PTPN14. Blakely WJ, Hatterschide J, White EA. mBio. 2024 Sep 9:e0181124. doi: 10.1128/mbio.01811-24. 10.1128/mbio.01811-24 PubMed 39248565
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