p8552 pLIX-PTPN14 ΔPPXY3/4
(Plasmid
#221645)
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PurposeDoxycycline-inducible overexpression of human PTPN14 ΔPPXY3/4 mutant
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLIX_402 GAW
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePTPN14 ΔPPXY3/4
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SpeciesH. sapiens (human)
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Mutationdeletion of the third and fourth PPXY motifs as described in PMID: 31486770
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Entrez GenePTPN14 (a.k.a. CATLPH, PEZ, PTP36, PTPD2)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.07.583953 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p8552 pLIX-PTPN14 ΔPPXY3/4 was a gift from Elizabeth White (Addgene plasmid # 221645 ; http://n2t.net/addgene:221645 ; RRID:Addgene_221645) -
For your References section:
HPV18 E7 inhibits LATS1 kinase and activates YAP1 by degrading PTPN14. Blakely WJ, Hatterschide J, White EA. mBio. 2024 Sep 9:e0181124. doi: 10.1128/mbio.01811-24. 10.1128/mbio.01811-24 PubMed 39248565