Skip to main content
Addgene

AAV-hSyn-ZipV-2A-IvfChr (citrine, KV2.1)
(Plasmid #221618)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    AAV
  • Backbone size w/o insert (bp) 4183
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1
  • Species
    Synthetic
  • Insert Size (bp)
    3258
  • Mutation
    ZipACR (I151V) with soma targeting, vfChrimson with membrane trafficking enhancement and soma targeting
  • Promoter Human Synapsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

soma targeted ZipACR (I151V) and 2A mediated expression of membrane optimized vfChrimson and soma targeting, citrine as vfChrimson expression marker.

Please visit https://doi.org/10.7554/eLife.90327.1 for preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-hSyn-ZipV-2A-IvfChr (citrine, KV2.1) was a gift from John Lin (Addgene plasmid # 221618 ; http://n2t.net/addgene:221618 ; RRID:Addgene_221618)

  • For your References section:

    Dual-color optical activation and suppression of neurons with high temporal precision. Mermet-Joret N, Moreno A, Zbela A, Ellendersen BE, Krauth N, von Philipsborn A, Piriz J, Lin JY, Nabavi S. eLife 2023 12:RP90327 10.7554/eLife.90327.1
Related items: