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Addgene

pEBG-hBEN-deltaNLS3
(Plasmid #22161)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBG
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hBEN
  • Alt name
    MusTRD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2900
  • Mutation
    Deletion of amino acids 883-889
  • Entrez Gene
    GTF2IRD1 (a.k.a. BEN, CREAM1, GTF3, MUSTRD1, RBAP2, WBS, WBSCR11, WBSCR12, hMusTRD1alpha1)
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer EF-1a Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBG-hBEN-deltaNLS3 was a gift from Ananda Roy (Addgene plasmid # 22161 ; http://n2t.net/addgene:22161 ; RRID:Addgene_22161)

  • For your References section:

    Repression of TFII-I-dependent transcription by nuclear exclusion. Tussie-Luna MI, Bayarsaihan D, Ruddle FH, Roy AL. Proc Natl Acad Sci U S A. 2001 Jul 3. 98(14):7789-94. 10.1073/pnas.141222298 PubMed 11438732
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