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Addgene

AAV-hSyn-PiGM-Iq(D387A eGFP)
(Plasmid #221603)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221603 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Backbone size w/o insert (bp) 4182
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PiGM-Iq (D387A, eGFP)
  • Species
    Synthetic
  • Insert Size (bp)
    3435
  • Mutation
    RGS2 1-53 truncation, CRYPHR D387A
  • Promoter Human Synapsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Negative control PiGM-Iq system using light insensitive CRY2PHR (D387A). Membrane EGFP as expression marker. hSyn promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-hSyn-PiGM-Iq(D387A eGFP) was a gift from John Lin (Addgene plasmid # 221603 ; http://n2t.net/addgene:221603 ; RRID:Addgene_221603)
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