OpenCRISPR-1 sgRNA-12nt stem
(Plasmid #221567)

• Purpose: Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
• Depositing Lab: Ali Madani
• Publication: Design of highly functional genome editors by modeling the universe of CRISPR-Cas sequences (unpublished)

Backbone

• Vector backbone: pTwist CMV
• Backbone manufacturer: Twist
• Backbone size w/o insert (bp): 4303
• Total vector size (bp): 5093
• Modifications to backbone: Removed repeated rrnG and T7 terminators to increase plasmid stability. Added GFP reporter cassette.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OpenCRISPR-1 sgRNA 12nt stem loop
• Species: H. sapiens (human)
• Insert Size (bp): 1788
• Promoter: U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Whole plasmid sequencing

Resource Information

• Supplemental Documents:
  • opencrispr-1-sgrna-12nt-stem.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA