OpenCRISPR-1
(Plasmid #221565)

• Purpose: Express OpenCRISPR-1 nuclease in human cells using a CMV promoter
• Depositing Lab: Ali Madani
• Publication: Design of highly functional genome editors by modeling the universe of CRISPR-Cas sequences (unpublished)

Backbone

• Vector backbone: pTwist CMV
• Backbone manufacturer: Twist
• Backbone size w/o insert (bp): 4304
• Total vector size (bp): 8172
• Modifications to backbone: Removed repeated rrnG and T7 terminators to increase plasmid stability
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OpenCRISPR-1
• Species: H. sapiens (human)
• Insert Size (bp): 4140
• Promoter: CMV
• Tag / Fusion Protein:
  • NLS (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Whole plasmid sequencing

Resource Information

• Supplemental Documents:
  • opencrispr-1-addgene-deposit.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA