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Addgene

pPB-EF1A-Puro-hTBK1-E696K-EGFP
(Plasmid #221554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB
  • Backbone manufacturer
    Vectorbuilder
  • Vector type
    Mammalian Expression ; piggybac
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TBK1
  • Species
    H. sapiens (human)
  • Mutation
    Changed glutamate 696 to lysine
  • Entrez Gene
    TBK1 (a.k.a. FTDALS4, IIAE8, NAK, T2K)
  • Promoter EF1a
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid was custom synthesized by Vectorbuilder

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-EF1A-Puro-hTBK1-E696K-EGFP was a gift from Shawn Ferguson (Addgene plasmid # 221554 ; http://n2t.net/addgene:221554 ; RRID:Addgene_221554)

  • For your References section:

    Lysosomal TBK1 Responds to Amino Acid Availability to Relieve Rab7-Dependent mTORC1 Inhibition. Talaia G, Bentley-DeSousa A, Ferguson SM. bioRxiv [Preprint]. 2023 Dec 17:2023.12.16.571979. doi: 10.1101/2023.12.16.571979. 10.1101/2023.12.16.571979 PubMed 38168426
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