pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459) Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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SnapGene

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> Addgene NGS Result ATCTCGTCCAGGTAGTGCTTGTGCTGTTCCACAAACAGCTGTTTCTGCTCATTATCCTCGGGGGAGCCCT TCAGCTTCTCATAGTGGCTGGCCAGGTACAGGAAGTTCACATATTTGGAGGGCAGGGCCAGTTCGTTTCC CTTCTGCAGTTCGCCGGCAGAGGCCAGCATTCTCTTCCGGCCGTTTTCCAGCTCGAACAGGGAGTACTTA GGCAGCTTGATGATCAGGTCCTTTTTCACTTCTTTGTAGCCCTTGGCTTCCAGAAAGTCGATGGGATTCT TCTCGAAGCTGCTTCTTTCCATGATGGTGATCCCCAGCAGCTCTTTCACACTCTTCAGTTTCTTGGACTT GCCCTTTTCCACTTTGGCCACCACCAGCACAGAATAGGCCACGGTGGGGCTGTCGAAGCCGCCGTACTTC TTAGGGTCCCAGTCCTTCTTTCTGGCGATCAGCTTATCGCTGTTCCTCTTGGGCAGGATAGACTCTTTGC TGAAGCCGCCTGTCTGCACCTCGGTCTTTTTCACGATATTCACTTGGGGCATGCTCAGCACTTTCCGCAC GGTGGCAAAATCCCGGCCCTTATCCCACACGATCTCCCCGGTTTCGCCGTTTGTCTCGATCAGAGGCCGC TTCCGGATCTCGCCGTTGGCCAGGGTAATCTCGGTCTTGAAAAAGTTCATGATGTTGCTGTAGAAGAAGT ACTTGGCGGTAGCCTTGCCGATTTCCTGCTCGCTCTTGGCGATCATCTTCCGCACGTCGTACACCTTGTA GTCGCCGTACACGAACTCGCTTTCCAGCTTAGGGTACTTTTTGATCAGGGCGGTTCCCACGACGGCGTTC AGGTAGGCGTCGTGGGCGTGGTGGTAGTTGTTGATCTCGCGCACTTTGTAAAACTGGAAATCCTTCCGGA AATCGGACACCAGCTTGGACTTCAGGGTGATCACTTTCACTTCCCGGATCAGCTTGTCATTCTCGTCGTA CTTAGTGTTCATCCGGGAGTCCAGGATCTGTGCCACGTGCTTTGTGATCTGCCGGGTTTCCACCAGCTGT CTCTTGATGAAGCCGGCCTTATCCAGTTCGCTCAGGCCGCCTCTCTCGGCCTTGGTCAGATTGTCGAACT TTCTCTGGGTAATCAGCTTGGCGTTCAGCAGCTGCCGCCAGTAGTTCTTCATCTTCTTCACGACCTCTTC GGAGGGCACGTTGTCGCTCTTGCCCCGGTTCTTGTCGCTTCTGGTCAGCACCTTGTTGTCGATGGAGTCG TCCTTCAGAAAGCTCTGAGGCACGATATGGTCCACATCGTAGTCGGACAGCCGGTTGATGTCCAGTTCCT GGTCCACGTACATATCCCGCCCATTCTGCAGGTAGTACAGGTACAGCTTCTCGTTCTGCAGCTGGGTGTT TTCCACGGGGTGTTCTTTCAGGATCTGGCTGCCCAGCTCTTTGATGCCCTCTTCGATCCGCTTCATTCTC TCGCGGCTGTTCTTCTGTCCCTTCTGGGTGGTCTGGTTCTCTCTGGCCATTTCGATCACGATGTTCTCGG GCTTGTGCCGGCCCATCACTTTCACGAGCTCGTCCACCACCTTCACTGTCTGCAGGATGCCCTTCTTAAT GGCGGGGCTGCCGGCCAGATTGGCAATGTGCTCGTGCAGGCTATCGCCCTGGCCGGACACCTGGGCTTTC TGGATGTCCTCTTTAAAGGTCAGGCTGTCGTCGTGGATCAGCTGCATGAAGTTTCTGTTGGCGAAGCCGT CGGACTTCAGGAAATCCAGGATTGTCTTGCCGGACTGCTTGTCCCGGATGCCGTTGATCAGCTTCCGGCT CAGCCTGCCCCAGCCGGTGTATCTCCGCCGCTTCAGCTGCTTCATCACTTTGTCGTCGAACAGGTGGGCA TAGGTTTTCAGCCGTTCCTCGATCATCTCTCTGTCCTCAAACAGTGTCAGGGTCAGCACGATATCTTCCA GAATGTCCTCGTTTTCCTCATTGTCCAGGAAGTCCTTGTCCTTGATAATTTTCAGCAGATCGTGGTATGT GCCCAGGGAGGCGTTGAACCGATCTTCCACGCCGGAGATTTCCACGGAGTCGAAGCACTCGATTTTCTTG AAGTAGTCCTCTTTCAGCTGCTTCACGGTCACTTTCCGGTTGGTCTTGAACAGCAGGTCCACGATGGCCT TTTTCTGCTCGCCGCTCAGGAAGGCGGGCTTTCTCATTCCCTCGGTCACGTATTTCACTTTGGTCAGCTC GTTATACACGGTGAAGTACTCGTACAGCAGGCTGTGCTTGGGCAGCACCTTCTCGTTGGGCAGGTTCTTA TCGAAGTTGGTCATCCGCTCGATGAAGCTCTGGGCGGAAGCGCCCTTGTCCACCACTTCCTCGAAGTTCC AGGGGGTGATGGTTTCCTCGCTCTTTCTGGTCATCCAGGCGAATCTGCTGTTTCCCCTGGCCAGAGGGCC CACGTAGTAGGGGATGCGGAAGGTCAGGATCTTCTCGATCTTTTCCCGGTTGTCCTTCAGGAATGGGTAA AAATCTTCCTGCCGCCGCAGAATGGCGTGCAGCTCTCCCAGGTGGATCTGGTGGGGGATGCTGCCGTTGT CGAAGGTCCGCTGCTTCCGCAGCAGGTCCTCTCTGTTCAGCTTCACGAGCAGTTCCTCGGTGCCGTCCAT CTTTTCCAGGATGGGCTTGATGAACTTGTAGAACTCTTCCTGGCTGGCTCCGCCGTCAATGTAGCCGGCG TAGCCGTTCTTGCTCTGGTCGAAGAAAATCTCTTTGTACTTCTCAGGCAGCTGCTGCCGCACGAGAGCTT TCAGCAGGGTCAGGTCCTGGTGGTGCTCGTCGTATCTCTTGATCATAGAGGCGCTCAGGGGGGCCTTGGT GATCTCGGTGTTCACTCTCAGGATGTCGCTCAGCAGGATGGCGTCGGACAGGTTCTTGGCGGCCAGAAAC AGGTCGGCGTACTGGTCGCCGATCTGGGCCAGCAGGTTGTCCAGGTCGTCGTCGTAGGTGTCCTTGCTCA GCTGCAGTTTGGCATCCTCGGCCAGGTCGAAGTTGCTCTTGAAGTTGGGGGTCAGGCCCAGGCTCAGGGC AATCAGGTTTCCGAACAGGCCATTCTTCTTCTCGCCGGGCAGCTGGGCGATCAGATTTTCCAGCCGTCTG CTCTTGCTCAGTCTGGCAGACAGGATGGCCTTGGCGTCCACGCCGCTGGCGTTGATGGGGTTTTCCTCGA ACAGCTGGTTGTAGGTCTGCACCAGCTGGATGAACAGCTTGTCCACGTCGCTGTTGTCGGGGTTCAGGTC GCCCTCGATCAGGAAGTGGCCCCGGAACTTGATCATGTGGGCCAGGGCCAGATAGATCAGCCGCAGGTCG GCCTTGTCGGTGCTGTCCACCAGTTTCTTTCTCAGGTGGTAGATGGTGGGGTACTTCTCGTGGTAGGCCA CCTCGTCCACGATGTTGCCGAAGATGGGGTGCCGCTCGTGCTTCTTATCCTCTTCCACCAGGAAGGACTC TTCCAGTCTGTGGAAGAAGCTGTCGTCCACCTTGGCCATCTCGTTGCTGAAGATCTCTTGCAGATAGCAG ATCCGGTTCTTCCGTCTGGTGTATCTTCTTCTGGCGGTTCTCTTCAGCCGGGTGGCCTCGGCTGTTTCGC CGCTGTCGAACAGCAGGGCTCCGATCAGGTTCTTCTTGATGCTGTGCCGGTCGGTGTTGCCCAGCACCTT GAATTTCTTGCTGGGCACCTTGTACTCGTCGGTGATCACGGCCCAGCCCACAGAGTTGGTGCCGATGTCC AGGCCGATGCTGTACTTCTTGTCGGCTGCTGGGACTCCGTGGATACCGACCTTCCGCTTCTTCTTTGGGG CCATCTTATCGTCATCGTCTTTGTAATCAATATCATGATCCTTGTAGTCTCCGTCGTGGTCCTTATAGTC CATGGTGGCACCGGTCCAACCTGAAAAAAAGTGATTTCAGGCAGGTGCTCCAGGTAATTAAACATTAATA CCCCACCAACCAACCATCCCTTAAACCCTTACCTCTTGCTCAGCTAATTACAGCCCGGAGGAGAAGGGCC GTCCCGCCCGCTCACCTGTGGGAGTAACGCGGTCAGTCAGAGCCGGGGCGGGCGGCGCGAGGCGGCGGCG GAGCGGGGCACGGGGCGAAGGCAGCGCGCAGCGACTCCCGCCCGCCGCGCGCTTCGCTTTTTATAGGGCC GCCGCCGCCGCCGCCTCGCCATAAAAGGAAACTTTCGGAGCGCGCCGCTCTGATTGGCTGCCGCCGCACC TCTCCGCCTCGCCCCGCCCCGCCCCTCGCCCCGCCCCGCCCCGCCTGGCGCGCGCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGGGGGGGGGGGGGGGGGGGGG GGGGGGGGGGGAAGCAGAAAGGGGGGCTCACCCCGCCCCGGGGAATAGCGATGACTAAAACGTAGATGTA CTTCCAAATAGGAAAGACCCCTAAAGTCATGTAATGGGGCACAATCCAGGGGGGGCCTTTTCCCTCATTT TCGTAAAAAGGGGGGGTAATTGGCCAATTATACACTTTATGTACTGCCAAGTGGGCAGTTTACCGTAAAT ACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATTGACGTCAATGGGCGGGGGTCGTT GGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGGGTACCTCTAGAGCCATTTGTCTGCAG AATTGGCGCACGCGCTAAAAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACAAAAAAG CACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCT AAAACGCTATCTAGGGTTTTGAATGCGGTGTTTCGTCCTTTCCACAAGATATATAAAGCCAAGAAATCGA AATACTTTCAAGTTACGGTAAGCATATGATAGTCCATTTTAAAACATAATTTTAAAACTGCAAACTACCC AAGAAATTATTACTTTCTACGTCACGTATTTTGTACTAATATCTTTGTGTTTACAGTCAAATTAATTCCA ATTATCTCTCTAACAGCCTTGTATCGTATATGCAAATATGAAGGAATCATGGGAAATAGGCCCTCACATG TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAG ATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAAC AGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA CTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGA AAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAG TTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCA CCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGA TACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGGCTTCCACGCTCACCGGCTCCAGA TTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCC ATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTG TTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCA ACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATC GTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTG TCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTAT GCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAA GTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTT CGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGC AAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTC TTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTA TTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAAC CATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGT GATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCG GGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGC ATCAGAGCAGATTGTACTGAGAGTGCACCATAAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTA AATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGACCGAAATCGGCAAAATCCCTTATAAATCAAAAG AATAGCCCGAGATAGAGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTC CAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCAAATCAAGT TTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGAC GGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAAGGCGCTGGC AAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTAC TATGGTTGCTTTGACGTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCG CCCCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACC TTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTT CCTGCGGCCGCTCCCCAGCATGCCTGCTATTCTCTTCCCAATCCTCCCCCTTGCTGTCCTGCCCCACCCC ACCCCCCAGAATAGAATGACACCTACTCAGACAATGCGATGCAATTTCCTCATTTTATTAGGAAAGGACA GTGGGAGTGGCACCTTCCAGGGTCAAGGAAGGCACGGGGGAGGGGCAAACAACAGATGGCTGGCAACTAG AAGGCACAGTCGAGGCTGATCAGCGAGCTCTAGTTAGAATTCTCAGGCACCGGGCTTGCGGGTCATGCAC CAGGTGCGCGGTCCTTCGGGCACCTCGACGTCGGCGGTGACGGTGAAGCCGAGCCGCTCGTAGAAGGGGA GGTTGCGGGGCGCGGAGGTCTCCAGGAAGGCGGGCACCCCGGCGCGCTCGGCCGCCTCCACTCCGGGGAG CACGACGGCGCTGCCCAGACCCTTGCCCTGGTGGTCGGGCGAGACTCCGACGGTGGCCAGGAACCACGCG GGCTCCTTGGGCCGGTGCGGCGCCAGGAGGCCTTCCATCTGTTGCTGCGCGGCCAGCCGGGAACCGCTCA ACTCGGCCATGCGCGGGCCGATCTCGGCGAACACCGCCCCCGCTTCGACGCTCTCCGGCGTGGTCCAGAC CGCCACCGCGGCGCCGTCGTCCGCGACCCACACCTTGCCGATGTCGAGCCCGACGCGCGTGAGGAAGAGT TCTTGCAGCTCGGTGACCCGCTCGATGTGGCGGTCCGGATCGACGGTGTGGCGCGTGGCGGGGTAGTCGG CGAACGCGGCGGCGAGGGTGCGTACGGCCCTGGGGACGTCGTCGCGGGTGGCGAGGCGCACCGTGGGCTT GTACTCGGTCATTGGGCCAGGATTCTCCTCGACGTCACCGCATGTTAGCAGACTTCCTCTGCCCTCTCCA CTGCCGAATTCCTTTTTCTTTTTTGCCTGGCCGGCCTTTTTCGTGGCCGCCGGCCTTTTGTCGCCTCCCA GCTGAGACAGGTCGATCCGTGTCTCGTACAGGCCGGTGATGCTCTGGTGGATCAGGGTGGCGTCCAGCAC CTCTTTGGTGCTGGTGTACCTCTTCCGGTCGATGGTGGTGTCAAAGTACTTGAAGGCGGCAGGGGCTCCC AGATTGGTCAGGGTAAACAGGTGGATGATATTCTCGGCCTGCTCTCTGATGGGCTTATCCCGGTGCTTGT TGTAGGCGGACAGCACTTTGTCCAGATTAGCGTCGGCCAGGATCACTCTCTTGGAGAACTCGCTGATCTG CTCGATG

pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459) Sequences (1)

Full Sequences from Addgene (1)

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