pEBG-TFII-I alpha
(Plasmid
#22154)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEBG
- Backbone size w/o insert (bp) 6100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTFII-I
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Alt nameTFII-I alpha isoform
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SpeciesH. sapiens (human)
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Insert Size (bp)3300
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Entrez GeneGTF2I (a.k.a. BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6)
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Tags
/ Fusion Proteins
- GST (N terminal on insert)
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site ClaI (unknown if destroyed)
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Restriction sites may not be correct.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEBG-TFII-I alpha was a gift from Ananda Roy (Addgene plasmid # 22154 ; http://n2t.net/addgene:22154 ; RRID:Addgene_22154) -
For your References section:
Alternatively spliced isoforms of TFII-I. Complex formation, nuclear translocation, and differential gene regulation. Cheriyath V, Roy AL. J Biol Chem. 2000 Aug 25. 275(34):26300-8. 10.1074/jbc.M002980200 PubMed 10854432
Map uploaded by the depositor.
