PSMC3_BirA_Nterm
(Plasmid
#221524)
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PurposeFlag-tagged biotinylation enzyme for co-expression of Psmc3
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBirA_Nterm
- Backbone size w/o insert (bp) 6189
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePSMC3
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SpeciesH. sapiens (human)
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Insert Size (bp)1320
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Entrez GenePSMC3 (a.k.a. DCIDP, RPT5, TBP1)
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Tag
/ Fusion Protein
- BirA (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PSMC3_BirA_Nterm was a gift from Alessandro Ori (Addgene plasmid # 221524 ; http://n2t.net/addgene:221524 ; RRID:Addgene_221524)