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Addgene

mSirt1-GFP
(Plasmid #221511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GFP-Cterm
  • Backbone size w/o insert (bp) 5845
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sirt1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1728
  • Entrez Gene
    Sirt1 (a.k.a. SIR2L1, Sir2, Sir2a, Sir2alpha)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mSirt1-GFP was a gift from Alessandro Ori (Addgene plasmid # 221511 ; http://n2t.net/addgene:221511 ; RRID:Addgene_221511)
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