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Addgene

pUbi_dCas9-BFP-KRAB-gRNA
(Plasmid #221503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME-Cas9-T2A-GFP
  • Vector type
    Fish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-tagBFP-KRAB
  • Species
    S. pyogenes
  • Mutation
    D10A and H839A point mutations
  • Promoter Zebrafish Ubiquitin promoter

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUbi_dCas9-BFP-KRAB-gRNA was a gift from Alessandro Ori (Addgene plasmid # 221503 ; http://n2t.net/addgene:221503 ; RRID:Addgene_221503)
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