pME-dCas9-BFP-KRAB
(Plasmid
#221502)
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PurposeCRISPRi machinery fish codon optimized
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221502 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepME-Cas9-T2A-GFP
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Vector typeFish Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9-tagBFP-KRAB
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SpeciesS. pyogenes
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MutationD10A and H839A point mutations
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-dCas9-BFP-KRAB was a gift from Alessandro Ori (Addgene plasmid # 221502 ; http://n2t.net/addgene:221502 ; RRID:Addgene_221502)