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Addgene

pME-dCas9-BFP-KRAB
(Plasmid #221502)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221502 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pME-Cas9-T2A-GFP
  • Vector type
    Fish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-tagBFP-KRAB
  • Species
    S. pyogenes
  • Mutation
    D10A and H839A point mutations

Cloning Information

  • Cloning method Other

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-dCas9-BFP-KRAB was a gift from Alessandro Ori (Addgene plasmid # 221502 ; http://n2t.net/addgene:221502 ; RRID:Addgene_221502)