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Addgene

pLenti_dCas9-KRAB-MeCP2_Blast
(Plasmid #221501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCas9-Blast
  • Backbone manufacturer
    Addgene #52962
  • Backbone size w/o insert (bp) 12859
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-KRAB-MeCP2-T2A-Blast
  • Species
    Synthetic
  • Insert Size (bp)
    5778
  • Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N863A
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CMV-w
  • 3′ sequencing primer WPRE-Rv
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_dCas9-KRAB-MeCP2_Blast was a gift from Matthew Meyerson (Addgene plasmid # 221501 ; http://n2t.net/addgene:221501 ; RRID:Addgene_221501)

  • For your References section:

    Dosage amplification dictates oncogenic regulation by the NKX2-1 lineage factor in lung adenocarcinoma. Pulice JL, Meyerson M. bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 10.1101/2023.10.26.563996. 10.1101/2023.10.26.563996 PubMed 37994369
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