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pLX-UBC_NKX2-1
(Plasmid #221495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221495 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX307
  • Backbone size w/o insert (bp) 10065
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NKX2-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1113
  • Entrez Gene
    NKX2-1 (a.k.a. BCH, BHC, NK-2, NKX2.1, NKX2A, NMTC1, T/EBP, TEBP, TITF1, TTF-1, TTF1)
  • Promoter UBC
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BmtI-HF (not destroyed)
  • 3′ sequencing primer WPRE-Rv
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UBC promoter was obtained from Luc.Cre empty vector (Addgene #20905) and cloned to replace EF1a promoter in pLX307 vector using PCR and restriction enzyme digestion/ligation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX-UBC_NKX2-1 was a gift from Matthew Meyerson (Addgene plasmid # 221495 ; http://n2t.net/addgene:221495 ; RRID:Addgene_221495)

  • For your References section:

    Dosage amplification dictates oncogenic regulation by the NKX2-1 lineage factor in lung adenocarcinoma. Pulice JL, Meyerson M. bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 10.1101/2023.10.26.563996. 10.1101/2023.10.26.563996 PubMed 37994369
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