pLX-EF1a_GFP_CdTag-HA
(Plasmid
#221492)
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PurposeGFP lentiviral overexpression of C-terminal dTag fusion
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221492 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLX307_CdTag-HA_EmptyVector
- Backbone size w/o insert (bp) 10378
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesSynthetic
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Insert Size (bp)742
- Promoter EF1a
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Tag
/ Fusion Protein
- FKBP F36V (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1a-Fw
- 3′ sequencing primer WPRE-Rv (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX-EF1a_GFP_CdTag-HA was a gift from Matthew Meyerson (Addgene plasmid # 221492 ; http://n2t.net/addgene:221492 ; RRID:Addgene_221492) -
For your References section:
Dosage amplification dictates oncogenic regulation by the NKX2-1 lineage factor in lung adenocarcinoma. Pulice JL, Meyerson M. bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 10.1101/2023.10.26.563996. 10.1101/2023.10.26.563996 PubMed 37994369
