pLX-EF1a_Empty
(Plasmid
#221480)
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Purpose(Empty Backbone) Empty vector control for pLX307 overexpresion experiments
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLX307
- Backbone size (bp) 10065
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Empty vector for pLX307 formed by BsrGI digestion to remove insert between att sites.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX-EF1a_Empty was a gift from Matthew Meyerson (Addgene plasmid # 221480 ; http://n2t.net/addgene:221480 ; RRID:Addgene_221480) -
For your References section:
Dosage amplification dictates oncogenic regulation by the NKX2-1 lineage factor in lung adenocarcinoma. Pulice JL, Meyerson M. bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 10.1101/2023.10.26.563996. 10.1101/2023.10.26.563996 PubMed 37994369
