pDONR223_mCherry
(Plasmid
#221470)
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PurposeGateway cloning of mCherry overexpression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR223
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5005
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Vector typeGateway Donor Vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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SpeciesSynthetic
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Insert Size (bp)708
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223_mCherry was a gift from Matthew Meyerson (Addgene plasmid # 221470 ; http://n2t.net/addgene:221470 ; RRID:Addgene_221470) -
For your References section:
Dosage amplification dictates oncogenic regulation by the NKX2-1 lineage factor in lung adenocarcinoma. Pulice JL, Meyerson M. bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 10.1101/2023.10.26.563996. 10.1101/2023.10.26.563996 PubMed 37994369