pLX311_CdTag-HA_EmptyVector
(Plasmid
#221468)
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Purpose(Empty Backbone) Gateway backbone for C-terminal dTag cloning
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221468 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLEX_305-C-dTAG
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Backbone manufacturerAddgene #91798
- Backbone size (bp) 9621
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
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Tag
/ Fusion Protein
- FKBP F36V (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- FKBP F36V (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX311_CdTag-HA_EmptyVector was a gift from Matthew Meyerson (Addgene plasmid # 221468 ; http://n2t.net/addgene:221468 ; RRID:Addgene_221468) -
For your References section:
Dosage amplification dictates oncogenic regulation by the NKX2-1 lineage factor in lung adenocarcinoma. Pulice JL, Meyerson M. bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 10.1101/2023.10.26.563996. 10.1101/2023.10.26.563996 PubMed 37994369
