lentiGuide_puro-P2A-mNeonGreen
(Plasmid #221464)

• Purpose: Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker
• Depositing Lab: James Eshleman
• Publication: lentiGuide_puro with mNeonGreen marker (unpublished)

Backbone

• Vector backbone: lentiGuide-puro-P2A-EGFP
• Backbone manufacturer: AddGene 137729
• Backbone size w/o insert (bp): 10963
• Total vector size (bp): 10954
• Modifications to backbone: Replaced GFP with mNeonGreen
• Vector type: Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mNeonGreen
• Species: Synthetic
• Insert Size (bp): 705
• Promoter: hU6, EF-1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: ACCGGTCgtgagcaagggcgaggag
• 3′ sequencing primer: ACGCGTcttgtacagctcgtccatg

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mNeonGreen was PCR amplified from Addgene Plasmid #122183

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry