insulator_seq_reporter_vector
(Plasmid
#221460)
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PurposeVector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221460 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneinsulator_seq_reporter_vector
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegypsy insulator-EGFP-neutral spacer DNA-mCherry
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
insulator_seq_reporter_vector was a gift from Maria Cristina Gambetta (Addgene plasmid # 221460 ; http://n2t.net/addgene:221460 ; RRID:Addgene_221460) -
For your References section:
Protocol for detecting genomic insulators in Drosophila using insulator-seq, a massively parallel reporter assay. Tonelli A, Cousin P, Gambetta MC. STAR Protoc. 2024 Oct 24;5(4):103391. doi: 10.1016/j.xpro.2024.103391. 10.1016/j.xpro.2024.103391 PubMed 39453817