pEBB-II-I-GFP alpha isoform
(Plasmid #22146)

• Depositing Lab: Ananda Roy
• Publication: Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-8.

Backbone

• Vector backbone: pEBB
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TFII-I
• Alt name: TFII-I alpha isoform
• Species: H. sapiens (human)
• Insert Size (bp): 2900
• Entrez Gene: GTF2I (a.k.a. BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: SnaB1 (unknown if destroyed)
• 5′ sequencing primer: EF-1a Forward
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The internal restriction sites may not be correct