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Addgene

2MLS-HyperNova/pcDNA3
(Plasmid #221457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
  • Alt name
    2MLS-HN
  • Species
    Synthetic
  • Insert Size (bp)
    900
  • GenBank ID
    LC771173

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2MLS-HyperNova/pcDNA3 was a gift from Kiwamu Takemoto (Addgene plasmid # 221457 ; http://n2t.net/addgene:221457 ; RRID:Addgene_221457)

  • For your References section:

    Optical inactivation of intracellular molecules by fast-maturating photosensitizing fluorescence protein, HyperNova. Shidara H, Shirai T, Ozaki-Noma R, Jitsuki S, Nagai T, Takemoto K. Commun Biol. 2024 Aug 6;7(1):945. doi: 10.1038/s42003-024-06583-x. 10.1038/s42003-024-06583-x PubMed 39107369
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