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pssAAV-2-hGFAP-GeNL-WPRE-bGHp(A)
(Plasmid #221455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pssAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Green enhanced Nano-lantern
  • Alt name
    GeNL
  • Species
    Synthetic
  • Insert Size (bp)
    1185
  • GenBank ID
    LC128715
  • Promoter hGFAP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CTCTGGGCACAGTGACCTC
  • 3′ sequencing primer GCAATAGGGAGGGGGAAAG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    GeNL was kindly provided by Takeharu Nagai.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pssAAV-2-hGFAP-GeNL-WPRE-bGHp(A) was a gift from Maiken Nedergaard (Addgene plasmid # 221455 ; http://n2t.net/addgene:221455 ; RRID:Addgene_221455)

  • For your References section:

    Oxygen imaging of hypoxic pockets in the mouse cerebral cortex. Beinlich FRM, Asiminas A, Untiet V, Bojarowska Z, Pla V, Sigurdsson B, Timmel V, Gehrig L, Graber MH, Hirase H, Nedergaard M. Science. 2024 Mar 29;383(6690):1471-1478. doi: 10.1126/science.adn1011. Epub 2024 Mar 28. 10.1126/science.adn1011 PubMed 38547288
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