pBAD33.lon
(Plasmid #22145)

• Depositing Lab: Robert Sauer
• Publication: Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

Backbone

• Vector backbone: pBad33
• Backbone manufacturer: Beckwith Lab
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lon
• Alt name: Protease La
• Species: E. coli K12
• Insert Size (bp): 2355
• Mutation: None, WT gene
• GenBank ID: EG10542
• Entrez Gene: lon (a.k.a. b0439, ECK0433, capR, deg, dir, lopA, muc)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xbal (not destroyed)
• 3′ cloning site: SphI (not destroyed)
• 5′ sequencing primer: pBad-fwd
• 3′ sequencing primer: pBad-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Eyal Gur
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry