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pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR
(Plasmid #221447)

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Item Catalog # Description Quantity Price (USD)
Plasmid 221447 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pHIV-NAT-T2A-hCD52
  • Backbone manufacturer
    Ralph Scully
  • Backbone size w/o insert (bp) 9173
  • Total vector size (bp) 10427
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Nourseothricin (clonNAT)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NFATC2IP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1254
  • Mutation
    SPR degron: hGEM (1-110) fragment that is fused to hSLBP (18-108), with GGGS linker in between. 1st Methionine of NFATC2IP deleted
  • Entrez Gene
    NFATC2IP (a.k.a. ESC2, NIP45, RAD60)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR was a gift from Daniel Durocher (Addgene plasmid # 221447 ; http://n2t.net/addgene:221447 ; RRID:Addgene_221447)

  • For your References section:

    NFATC2IP is a mediator of SUMO-dependent genome integrity. Cho T, Hoeg L, Setiaputra D, Durocher D. Genes Dev. 2024 Apr 17;38(5-6):233-252. doi: 10.1101/gad.350914.123. 10.1101/gad.350914.123 PubMed 38503515
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