ClpP-his
(Plasmid #22144)

• Depositing Lab: Robert Sauer
• Publication: Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Backbone

• Vector backbone: pQE70
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3426
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Expressed from Bl21 ClpP- (chlor), grown in LB with amp (200 ug/ml) at 37 C.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ClpP-His6
• Alt name: ClpP wild type
• Alt name: C-terminal His 6
• Species: E. Coli
• Insert Size (bp): 900
• Mutation: C-terminal His tag
• Tag / Fusion Protein:
  • his (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: unknown (see pQE70 map)
• 3′ sequencing primer: CAT-R (?)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This was acquired from Dr. Andreas Martin, UC Berkeley

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NGS results indicate S2P in ClpP