pENTR-ABCC1_S769N_STOP
(Plasmid
#221431)
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PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR
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Backbone manufacturerTwist Bioscience
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameABCC1
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SpeciesH. sapiens (human)
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Insert Size (bp)4593
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MutationS769N
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Entrez GeneABCC1 (a.k.a. ABC29, ABCC, DFNA77, GS-X, MRP, MRP1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13
- 3′ sequencing primer M13r (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
RESOLUTE ID: PD05PE-E
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-ABCC1_S769N_STOP was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 221431 ; http://n2t.net/addgene:221431 ; RRID:Addgene_221431)