pENTR-Sensor_P2A_SLC2A4
(Plasmid
#221429)
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PurposeGateway entry clone encoding a polyprotein that is cleaved to FRET glucose sensor and SLC2A4 proteins
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221429 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR
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Backbone manufacturerTwist Bioscience
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSensor_P2A_SLC2A4
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SpeciesSynthetic
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Insert Size (bp)3969
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Entrez GeneSLC2A4 (a.k.a. GLUT4)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13
- 3′ sequencing primer M13r (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFRET glucose sensor is described in: https://pubmed.ncbi.nlm.nih.gov/18177733/
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
RESOLUTE ID: PD056E-A
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-Sensor_P2A_SLC2A4 was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 221429 ; http://n2t.net/addgene:221429 ; RRID:Addgene_221429) -
For your References section:
The RESOLUTE consortium: unlocking SLC transporters for drug discovery. Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov V, Wolf G, Crawford M, Everley R, ... Steppan CM. Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 10.1038/d41573-020-00056-6. 10.1038/d41573-020-00056-6 PubMed 32265506