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Addgene

pEGFP-C1-A20
(Plasmid #22141)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22141 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    A20
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2373
  • Entrez Gene
    TNFAIP3 (a.k.a. A20, AIFBL1, AISBL, OTUD7C, TNFA1P2)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Hong-bing Shu Wuhan University China
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-A20 was a gift from Yihong Ye (Addgene plasmid # 22141 ; http://n2t.net/addgene:22141 ; RRID:Addgene_22141)

  • For your References section:

    Localization of A20 to a lysosome-associated compartment and its role in NFkappaB signaling. Li L, Hailey DW, Soetandyo N, Li W, Lippincott-Schwartz J, Shu HB, Ye Y. Biochim Biophys Acta. 2008 Jun . 1783(6):1140-9. 10.1016/j.bbamcr.2008.01.029 PubMed 18329387
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