Talin1 head EGFP fusion
(Plasmid
#221408)
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PurposeMammalian expression of Talin1 head EGFP fusion for mammalian expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221408 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFPN1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTalin1 head EGFP fusion
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SpeciesH. sapiens (human)
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Entrez GeneTLN1 (a.k.a. ILWEQ, TLN, talin-1)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Talin1 head EGFP fusion was a gift from Timothy Springer (Addgene plasmid # 221408 ; http://n2t.net/addgene:221408 ; RRID:Addgene_221408) -
For your References section:
Ligand binding initiates single-molecule integrin conformational activation. Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, Springer TA. Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049. 10.1016/j.cell.2024.04.049 PubMed 38772370