Beta1 N391* full-length
(Plasmid
#221404)
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PurposeMammalian expression of human integrin beta1 N391* full-length
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221404 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepD2529 CAG
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Backbone manufacturer15
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameintegrin beta1 N391* full-length
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SpeciesH. sapiens (human)
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Mutationcodon-optimized mature sequence
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Entrez GeneITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
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Tags
/ Fusion Proteins
- CD33 secretion peptide (MPLLLLLPLLWAGALA) (N terminal on insert)
- HA and AVI tags (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Beta1 N391* full-length was a gift from Timothy Springer (Addgene plasmid # 221404 ; http://n2t.net/addgene:221404 ; RRID:Addgene_221404) -
For your References section:
Ligand binding initiates single-molecule integrin conformational activation. Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, Springer TA. Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049. 10.1016/j.cell.2024.04.049 PubMed 38772370