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Addgene

Beta1 N391* N562* ectodomain
(Plasmid #221400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pD2529 CAG
  • Backbone manufacturer
    72
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    integrin beta1 N391* N562* ectodomain
  • Species
    H. sapiens (human)
  • Mutation
    codon optimized for human mature _1 residues Q1 to D708
  • Entrez Gene
    ITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
  • Tags / Fusion Proteins
    • CD33 secretion peptide (MPLLLLLPLLWAGALA) (N terminal on insert)
    • AVI tag, HRV3C cleavage site, basic coil, HA tag, and deca-histidine tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Beta1 N391* N562* ectodomain was a gift from Timothy Springer (Addgene plasmid # 221400 ; http://n2t.net/addgene:221400 ; RRID:Addgene_221400)

  • For your References section:

    Ligand binding initiates single-molecule integrin conformational activation. Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, Springer TA. Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049. 10.1016/j.cell.2024.04.049 PubMed 38772370
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