pcDNA3.4 human alpha5 full
(Plasmid
#221396)
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PurposeMammalian expression of human integrin alpha5 full length
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221396 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.4
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Backbone manufacturer11
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameintegrin alpha5 full length
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SpeciesH. sapiens (human)
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Entrez GeneITGA5 (a.k.a. CD49e, FNRA, VLA-5, VLA5A)
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Tags
/ Fusion Proteins
- CD33 secretion peptide (MPLLLLLPLLWAGALA) (N terminal on insert)
- Strep twin (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.4 human alpha5 full was a gift from Timothy Springer (Addgene plasmid # 221396 ; http://n2t.net/addgene:221396 ; RRID:Addgene_221396) -
For your References section:
Ligand binding initiates single-molecule integrin conformational activation. Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, Springer TA. Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049. 10.1016/j.cell.2024.04.049 PubMed 38772370