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Addgene

pcDNA3.4 human alpha5 full
(Plasmid #221396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221396 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.4
  • Backbone manufacturer
    11
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    integrin alpha5 full length
  • Species
    H. sapiens (human)
  • Entrez Gene
    ITGA5 (a.k.a. CD49e, FNRA, VLA-5, VLA5A)
  • Tags / Fusion Proteins
    • CD33 secretion peptide (MPLLLLLPLLWAGALA) (N terminal on insert)
    • Strep twin (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.4 human alpha5 full was a gift from Timothy Springer (Addgene plasmid # 221396 ; http://n2t.net/addgene:221396 ; RRID:Addgene_221396)

  • For your References section:

    Ligand binding initiates single-molecule integrin conformational activation. Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, Springer TA. Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049. 10.1016/j.cell.2024.04.049 PubMed 38772370
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