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PD2529 human alpha5 ecto
(Plasmid #221394)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221394 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pD2529 CAG
  • Backbone manufacturer
    65
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    integrin alpha5 ectodomain
  • Species
    H. sapiens (human)
  • Mutation
    codon optimized for human mature residues _5 F1 to Y954
  • Entrez Gene
    ITGA5 (a.k.a. CD49e, FNRA, VLA-5, VLA5A)
  • Tags / Fusion Proteins
    • CD33 secretion peptide (MPLLLLLPLLWAGALA) (N terminal on insert)
    • HRV3C cleavage site, acid coil, Protein C tag, and Strep twin tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PD2529 human alpha5 ecto was a gift from Timothy Springer (Addgene plasmid # 221394 ; http://n2t.net/addgene:221394 ; RRID:Addgene_221394)

  • For your References section:

    Ligand binding initiates single-molecule integrin conformational activation. Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, Springer TA. Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049. 10.1016/j.cell.2024.04.049 PubMed 38772370
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