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Addgene

pcDNA5-FRT/T0-3XFLAG-NFATC2IP SLD2
(Plasmid #221388)

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Item Catalog # Description Quantity Price (USD)
Plasmid 221388 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcDNA5-3X-Flag-FRT-T0
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 5191
  • Total vector size (bp) 5416
  • Modifications to backbone
    1xFLAG was replaced by HindIII-3xFLAG-AscI, yielding 3xFLAG vector for N-terminal tagging with MCS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFATC2IP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    225
  • Mutation
    Amino acids 1-344 of NFATC2IP deleted
  • Entrez Gene
    NFATC2IP (a.k.a. ESC2, NIP45, RAD60)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3XFlag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT/T0-3XFLAG-NFATC2IP SLD2 was a gift from Daniel Durocher (Addgene plasmid # 221388 ; http://n2t.net/addgene:221388 ; RRID:Addgene_221388)

  • For your References section:

    NFATC2IP is a mediator of SUMO-dependent genome integrity. Cho T, Hoeg L, Setiaputra D, Durocher D. Genes Dev. 2024 Apr 17;38(5-6):233-252. doi: 10.1101/gad.350914.123. 10.1101/gad.350914.123 PubMed 38503515
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